Metabolic Core Lab
The Metabolic Core Lab is available for both academic and non-academic investigators.
For more information please contact:
Ted Ciaraldi, PhD
tciaraldi@ucsd.edu
858-552-8585 ext. 6450
Cell Culture:
- Provide excess cultured human skeletal muscle cells from metabolically well
characterized subjects for use as a tool to rapidly and effectively evaluate the
biologic activity of new compounds in human muscle. - Prepare and maintain primary cultures of cells from human tissues.
- Maintain adipose tissue under defined conditions to test activities of new compounds.
Adipose Tissue:
Isolate adipocytes from adipose tissue.
Metabolic Assays:
Perform assays of metabolic flux in human adipocytes and muscle cells,
to test activities of new compounds or determine the impact of different
pathophysiologies.
- Glucose uptake
- Fatty acid oxidation
Biologic Assays:
- Pyrogenicity testing of labeled compounds prior to infusion.
- Determination of glucose-specific activity in serum from individuals infused
with 3H-glucose; calculate the rate of hepatic glucose production. - Multiplex analysis of cytokines and inflammatory factors in samples:
plasma, serum, or culture media.